Method to achieve a standard linear curve in a sandwich...

Details Method to achieve a standard linear curve in a sandwich... Method to achieve a standard linear curve in a sandwich...

G - Physics

01

N

167/41

G01N 33/543 (2006.01) G01N 33/68 (2006.01) G01N 33/74 (2006.01)

Patent

CA 1294870

METHOD TO ACHIEVE A LINEAR STANDARD CURVE IN A SANDWICH IMMUNOASSAY Abstract A method for obtaining an actual linear standard curve in a sandwich type of immunoassay where a first antibody bound to an insoluble support and a second unbound labelled antibody complex with the antigen contained in a test sample to form an insoluble antibody: antigen:labelled antibody complex which is then detected. Unbound unlabelled first antibody and/or unbound unlabelled second antibody are added to the reaction mixture to divert excess antigen away from the desired end-product complex, thus rendering the antigen of interest the rate-limiting factor in the overall immunoreaction. This results in a pseudo first-order reaction which produces an actual linear standard curve.

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