C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
530/13, 195/39.1
C12N 15/55 (2006.01) C07K 14/515 (2006.01) C12N 1/21 (2006.01) C12N 9/64 (2006.01) C12N 15/18 (2006.01) A61K 38/00 (2006.01)
Patent
CA 1331356
ABSTRACT Site-specific mutagenesis of a gene for angiogenin producing DNA sequences encoding mutant proteins having increased angiogenic activity are disclosed. Expression vectors containing these sequences are introduced into host cells and direct the production of the mutant angiogenic proteins with markedly increased angiogenic and ribonucleolytic activity. Replacement of a single amino acid, the aspartic acid at or corresponding to position 116 of angiogenin, with another amino acid including asparagine, alanine or histidine, yields mutant proteins with 8 to 15 fold increased ribonucleolytic activity toward tRNA and rRNA and 10 to 100 fold increased angiogenic potency in the chorioallantoic membrane assay. The mutant angiogenin proteins of this invention are useful therapeutic compositions to promote the development of a hemovascular network in a mammal or to promote wound healing, in particular, healing of torn or traumatized fibrocartilage material.
594729
Harper Jeffrey W.
Vallee Bert L.
Predident And Fellows Of Harvard College
Smart & Biggar
LandOfFree
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