Method and kit for assaying gene expressions

Details Method and kit for assaying gene expressions Method and kit for assaying gene expressions

C - Chemistry, Metallurgy

12

Q

150/8.5

C12Q 1/68 (2006.01) G01N 33/58 (2006.01)

Patent

CA 1321746

A B S T R A C T A method of assaying gene expressions comprises linking mRNA molecules, including certain target mRNA molecules to be assayed, to a solid substrate; contacting said solid substrate with an aqueous suspension of labelled microbeads to which gene probe molecules are linked, said gene probe molecules comprising sequences which hybridize with sequences comprised by and characteristic of said certain target mRNA molecules to be assayed, thereby selectively linking said labelled microbeads to said certain target mRNA molecules to be assayed which are linked to said substrate; separating the solid substrate from microbeads which are not linked to said target mRNA molecules; and determining the presence or quantity of said labelled, linked microbeads: thereby selectively assaying said gene expressions.

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