Construction of novel mutant microorganisms

Details Construction of novel mutant microorganisms Construction of novel mutant microorganisms

C - Chemistry, Metallurgy

12

N

195/49, 195/34.4

C12N 1/20 (2006.01) C12N 9/02 (2006.01) C12N 15/52 (2006.01) C12P 7/42 (2006.01)

Patent

CA 1237997

ABSTRACT OF THE DISCLOSURE This invention provides novel mutant strains of microorganisms (e.g., Pseudomonas putida Biotype A) which are capable of converting substrates such as toluene, p-xylene, catechol and 4-methylcatechol to 2-hydroxymuconic semialdehyde or substituted analog of 2-hydroxymuconic semialdehyde quantitatively by the meta (catechol 2,3-oxygenase) pathway. No active 2-hydroxymuconic semialdehyde-metabolizing enzymes are induced in the microorganism, thereby permitting a 2-hydroxymuconic semialdehyde type metabolite to be produced and accumulated in a bioconversion medium containing the microorganism.

463173

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