Process and medium for culturing ant venom gland cells

Details Process and medium for culturing ant venom gland cells Process and medium for culturing ant venom gland cells

C - Chemistry, Metallurgy

12

N

195/46, 195/34.6

C12N 5/00 (2006.01) A61K 35/64 (2006.01) C12N 5/06 (2006.01) C12P 1/00 (2006.01)

Patent

CA 1212644

PROCESS AND MEDIUM FOR CULTURING ANT VENOM GLANDS Abstract of Disclosure In vitro culture of the venom gland cells of the ant Pseudomyremex triplarinus is effected by removing venom gland tissue from a larva or pupa of P. triplarinus subjecting the tissue to a protease to separate out the cells therein and culturing the resultant cells in an isotonic saline medium comprising carbohydrate, amino acids guanosine 3',5'-cyclic monophosphate and adenosine 3',5'-cyclic monophosphoric acid, at least part of the medium having been conditioned with Spodoptera frugiperda cells. Such in vitro culture provides a practicable route to volume production of the biologically active materials which are useful for treating the symptoms of rheurnatoid arthritis and which the venom gland cells are known to produce.

463194

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