Electrokinetic separation of chiral compounds

B - Operations – Transporting – 01 – D

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204/99.01

B01D 53/32 (2006.01)

Patent

CA 1309059

-32- Abstract of the Invention An electrokinetic process and apparatus employing coherent radiation-excited fluorescence for detection is disclosed. Specifically, the detection of electrokine- tically-transported target species as said species pass in a support electrolyte through a detection volume can be carried out with improved efficiency when the detection event asso- ciated with the passage of the species through the detection volume is a change in emitted light, in particular fluores- cence, which emitted light has been generated by a beam of electromagnetic radiation supplied on-column by a coherent source. The use of coherent radiation source permits the radiation of a well-defined wavelength to be delivered on- column to the sample without hazard and without appreciable loss of intensity and without unwanted interference from scattered light, as compared to incoherent light sources. The use of coherent radiation increases sensitivity because interference from Raman and Rayleigh scattering is minimized. This detection system allows mixtures of compounds to be analyzed and/or separation with improved efficiency. It is possible, using the detection system of this invention, to detect amount of targets in the range of a femtomole (10-15 moles) or less.

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