Process for transformation of yarrowia lipolytica

Details Process for transformation of yarrowia lipolytica Process for transformation of yarrowia lipolytica

C - Chemistry, Metallurgy

12

N

195/1.15, 195/1.

C12N 15/00 (2006.01) C12N 1/16 (2006.01) C12N 9/60 (2006.01) C12N 15/81 (2006.01) C12N 15/90 (2006.01)

Patent

CA 1226234

PROCESS FOR TRANSFORMATION OF YARROWIA LIPOLYTICA Abstract Process for transformation of Yarrowia lipolytica, vectors useful therefor comprising DNA of a microbial vector and chromosomal DNA of Y. lipolytica and transformants comprising said vectors in E. coli and Y. lipolytica, and integrative shuttle vectors for Escherichia-Yarrowia transgeneric cloning. Said vectors or subclones thereof enable creation of Y. lipolytica, cloning vectors into which specific or random segments of DNA can be inserted and the resulting vectors used to transform a suitable host microbe, especially Y. lipolytica, to improve the fermentation charactsristics thereof and hence their industrial utilization. The methodology described permits the cloning of genes from a gene library of Y.lipolytica by complementation with an integrating vector.

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