G - Physics – 01 – N
Patent
G - Physics
01
N
167/37
G01N 33/537 (2006.01)
Patent
CA 1202235
ABSTRACT A method for removing complement components C3, C4 and C5 from samples of biological fluids and recovering from the fluids complement fragments C3a, C4a and C5a or the des-Arg derivatives thereof, which com- prises: combining equal volumes of the biological fluid sample and a buffered solution of 0.8 to 1.6% of an acridine derivative selected from the group consisting of acrinol, acriflavine, acriflavine hydrochloride and aminacrine; incubating the mixture for about one minute to 2 hours at about 25°C; and recovering the supernatant, containing the complement fragments, from the resultant precipitate. Also a method for assaying the complement fragments, which comprises: incubating the supernatant with a known amount of a labeled complement fragment C3a, C4a or C5a, or the des-Arg derivative thereof and a known amount of antibody which recognizes the complement fragment or the des-Arg derivative thereof; separating the free labeled complement fragment from the bound labeled complement fragment; measuring the amount of labeled complement fragment in either the free or antibody bound complement component; and determining the concen- tration of complement fragment or the des-Arg derivative thereof in the biological sample by comparison to a standard curve. A kit for effecting the above processes is also disclosed.
435635
Macrae & Co.
Upjohn Company (the)
LandOfFree
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