C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
167/130, 195/1.2
C12N 15/54 (2006.01) A61K 39/245 (2006.01) C07K 14/03 (2006.01) C07K 14/15 (2006.01) C12N 7/01 (2006.01) C12N 15/00 (2006.01) C12N 15/10 (2006.01) C12N 15/86 (2006.01) C12N 15/869 (2006.01) C12Q 1/68 (2006.01) A61K 38/00 (2006.01)
Patent
CA 1340473
Methods for isolating thymidine kinase-encoding DNA of a herpes virus are described. These methods utilize degenerate primers based on regions of relatively conserved amino acid sequence in herpes virus thymidine kinase proteins to initiate a polymerase chain reaction which yields large amounts of the thymidine kinase-encoding DNA. The methods are illustrated in the isolation of the thymidine kinase gene of feline herpes virus, which can be used to construct recombinant thymidine kinase-negative feline herpes viruses for purposes of constructing live vaccines and expression vectors. In addition, the regulatory elements of the feline herpes virus thymidine kinase gene are useful in the construction of recombinant DNA vectors.
607697
Compton Teresa
Nunberg H. Jack
Petrovskis A. Erik
Post E. Leonard
Cetus Corporation
Macrae & Co.
The Upjohn Company
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