Process for recovering glucagon from pancreas glands

Details Process for recovering glucagon from pancreas glands Process for recovering glucagon from pancreas glands

C - Chemistry, Metallurgy

07

K

530/7.34

C07K 14/605 (2006.01)

Patent

CA 1284000

Abstract There is provided a process for the purifi- cation of glucagon from a glucagon salt cake containing pancreas gland-sourced glucagon, which comprises (1) dissolving said glucagon salt cake in an aqueous medium containing a water-miscible organic solvent; (2) contacting the glucagon salt cake solution with a hydrophobic adsorption support at a pH less than about 4 or greater than about 7.5 and at conditions under which the glucagon is adsorbed on said hydrophobic adsorption support; (3) eluting glucagon from said hydrophobic adsorp- tion support with an aqueous solution contain- ing a water-miscible organic solvent to obtain a first glucagon-containing solution; (4) contacting a solution containing glucagon eluted from said hydrophobic adsorption support, said solution having a pH of from about 7.5 to about 10.5, with an anion exchange support at conditions under which the glucagon is adsorbed on said anion exchange support; (5) eluting glucagon from said anion exchange support to obtain a second glucagon-contain- ing solution; and (6) recovering glucagon having increased purity.

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