Drug screening assay

Details Drug screening assay Drug screening assay

G - Physics

01

N

167/45

G01N 33/567 (2006.01) G01N 33/535 (2006.01) G01N 33/78 (2006.01) G01N 33/82 (2006.01) G01N 33/94 (2006.01)

Patent

CA 1335707

Compounds are evaluated for their binding to naturally occurring receptors, by employing the natural ligand conjugated to an enzyme donor fragment of .beta.- galactosidase for competing with the sample compound for the natural acceptor binding site or in the absence of competition where the sample compound binds to an allosteric site. By adding the enzyme acceptor fragment of the .beta.-galactosidase and substrate, the binding affinity of the sample compound may be evaluated as a measure of agonist or antagonist capability.

613938

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