C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
530/13, 530/15.1
C07K 14/765 (2006.01) C30B 7/00 (2006.01)
Patent
CA 1336530
HSA crystals are provided in the form of tetragonal plates having the space groups P4212, the crystals being grown to sizes in excess of 0.5 mm in two dimensions and a thickness of 0.1 mm. Growth of the crystals is carried out by hanging drop method wherein a precipitant solution containing PEG and a phosphate buffer is mixed with an HSA solution, and a droplet of mixed solution is suspended over a well of precipitant solution. Crystals grow to the desired size in 3 to 7 days. Concen- tration of reagents, pH and other parameters are con- trolled within prescribed limits. The resulting crystals exhibit a size and quality such as to allow performance of x-ray diffraction studies and enable the conduct of drug binding studies as well as genetic engineering studies.
575341
Battison Williams Dupuis
National Aeronautics And Space Administration
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