Transformed myeloma cell-line and a process for the...

Details Transformed myeloma cell-line and a process for the... Transformed myeloma cell-line and a process for the...

C - Chemistry, Metallurgy

12

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195/1.29, 195/1.

C12N 15/58 (2006.01) C12N 5/10 (2006.01) C12N 9/72 (2006.01) C12N 15/12 (2006.01) C12N 15/85 (2006.01)

Patent

CA 1319120

- 27 - ABSTRACT TRANSFORMED MYELOMA CELL-LINE AND A PROCESS FOR THE EXPRESSION OF A GENE CODING FOR A EURKARYOTIC POLYPEPTIDE EMPLOYING SAME A myeloma cell-line transformed with a vector including a gene coding for a eukaryotic polypeptide and a non-immunoglobulin promoter such that expression occurs of the gene coding for the eukaryotic polypeptide, directed by the non-immunoglobulin promoter. The promoter may be a viral promoter, such as an SV40 promoter, a Rous sarcoma virus long terminal repeat or a Moloney murine leukemia long terminal repeat, or a non-viral promoter such as the mouse metallothionein promoter. Rat and mouse host myeloma cell-lines such as the rat YB2/3.0 Ag20 hybridoma, the mouse SP2-O Ag hybridoma and the mouse NSO hybridoma are employed. The production of tissue plasminogen activator (tPA) is exemplified.

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