Serum-free culture medium and process for making same

C - Chemistry – Metallurgy – 12 – N

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C12N 5/02 (2006.01) C12N 5/00 (2006.01)

Patent

CA 1209070

SERUM-FREE CELL CULTURE MEDIUM AND PROCESS FOR MAKING SAME Abstract A defined serum-free medium that is capable of growing a wide range of suspension and monolayer cells in- cludes a serum substitute composed of fetuin, transferrin, 1-oleoyl-2-palmitoyl-phosphatidylcholine, linoleic acid and cholesterol. The medium also includes various inorganic salts, carbohydrates, amino acids, buffering agents, vitamins, and compounds to stimulate the natural cell environment.

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