Vectors

Details Vectors Vectors

C - Chemistry, Metallurgy

12

N

195/1.2, 195/1.2

C12N 15/81 (2006.01) C12N 1/19 (2006.01) C12N 15/11 (2006.01) C12N 15/85 (2006.01)

Patent

CA 1281672

43 ABSTRACT Vectors A eukaryotic expression vector includes a controllable repressor operator sequence within the expression control sequence of the vector. The controllable repressor operator sequence represses expression of a heterologous structural gene located downstream of the expression control sequence in the presence of a gene product or a combination of gene products of a yeast mating type locus. This allows control of the expression level of the heterologous structural gene. Controllable repressor operator sequences responsive to the al/.alpha.2 and .alpha.2 gene products of the MATa and MAT yeast mating type locus alleles are described, both derived from material and synthesised chemically.

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