Nucleic acid hybridization assay employing immobilized rna...

Details Nucleic acid hybridization assay employing immobilized rna... Nucleic acid hybridization assay employing immobilized rna...

G - Physics

01

N

150/3, 150/8.5

G01N 33/53 (2006.01) C12Q 1/68 (2006.01) G01N 33/543 (2006.01)

Patent

CA 1253777

ABSTRACT OF THE DISCLOSURE A nucleic acid hybridization assay employing an immobilized or immobilizable polynucleotide probe selected to form DNA-RNA or RNA-RNA hybrids with the particular polynucleotide sequence to be determined. Resulting hybrids are detected by binding of an antibody reagent, preferably labeled with a detectable chemical group, selective for binding the hybrids in the presence of the single stranded sample and probe nucleic acids. No immobilization or labeling of sample nucleic acids is necessary and hybridization can be performed entirely in solution.

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