Method of gene transfer into plants

C - Chemistry – Metallurgy – 12 – N

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C12N 15/84 (2006.01) A01H 1/00 (2006.01) A01H 4/00 (2006.01) C12N 5/04 (2006.01) C12N 15/82 (2006.01) C12N 15/87 (2006.01)

Patent

CA 1327173

Abstract of the Disclosure Method for gene transfer into plants, which com- prises a) isolation, from anthers, of immature pollen grains in nutrient solution and removing the tissue in which- they are embedded, b) culturing the isolated, immature pollen grains in a nutrient solution c) transferring foreign genetic material into the pol- len grains during the in vitro culturing and saturation d) bringing about complete saturation of the transformed pollen grains in vitro e) pollinating receiver plants with the transformed pollen grains and obtaining seds from the former and transgenic seeds and propagation products therefrom.

571824

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