Methods and compositions for detection of legionnaires' disease

Details Methods and compositions for detection of legionnaires' disease Methods and compositions for detection of legionnaires' disease

C - Chemistry, Metallurgy

12

N

195/1.39

C12N 15/00 (2006.01) C07K 14/195 (2006.01) C12N 1/20 (2006.01) G01N 33/531 (2006.01) G01N 33/569 (2006.01)

Patent

CA 1236413

METHODS AND COMPOSITIONS FOR DETECTION OF LEGIONNAIRES' DISEASE ABSTRACT Recombinant E. coli clones which cell surface-express Legionella pneumophila antigens, a method for utilizing these clones for the detection of Legionella antibodies in a clinical sample, and a method for isolation of mono- specific anti-Legionella antibodies are disclosed. The recombinant clones are produced by ligating fragmented Legionella DNA to pBR322 which is then used to transform the appropriate E. coli host. Clones that cell surface- express individual Legionella antigens are selected by screening cellularly intact clones using anti-Legionella antibodies to probe for cell surface expression. An enzyme-linked immunosorbant assay (ELISA) is disclosed which utilizes the Legionella antigen-expressing clones to detect anti-Legionella antibodies.

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