C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/8.5
C12Q 1/68 (2006.01) C12Q 1/02 (2006.01)
Patent
CA 1322943
ABSTRACT OF THE INVENTION. There is provided a process for screening an agent in order to determine whether such agent increases the frequency of genome rearrangement in living matter. In the first step of this process, there is provided a viable species of Saccharomyces cerevisiae yeast which comprises repeated genetic elements in its haploid genome. These repeated genetic elements are selected from the group consisting of functional and non-functional genetic elements; and these elements are sufficiently homologous so that, under ambient conditions, they recombine with each other and give rise to an identifiable genome rearrangement which is a deletion. In the second step of this process, the viable species of yeast is exposed to the agent to be tested. Thereafter, it is plated onto a growth medium which, after the exposed yeast species grows upon it, facilitates the identification of those yeast which have undergone said genome rearrangment. In the last step of the process, the extent to which the exposed species of yeast has undergone genome rearrangment is determined. Also disclosed is a the viable yeast strain used in said process, the plasmid used to construct said strain, and a process for constructing said strain. 46
586711
Genebiomed Inc.
Sim & Mcburney
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