Device and method for performing qualitative enzyme...

G - Physics – 01 – N

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167/44, 128/93

G01N 33/573 (2006.01) G01N 33/543 (2006.01) G01N 33/76 (2006.01)

Patent

CA 1230552

Abstract The invention relates to a device and method for performing qualitative enzyme immunoassays. The device comprises at least one tube having antibody, antigen or hapten attached to an internal surface thereof. A first syringe is connectable to a first end of the tube or tubes and supplies a flow of test liquid therethrough. A second syringe having a two piston arrangement is connected to the tube so as to allow a first wash solution followed by an enzyme conjugate solution to be flowed there- through. Finally a third syringe, also having a two piston configuration, is connected to the tube so as to allow a second wash solution followed by an enzyme substrate and metabolite indicator solution to be flowed therethrough.

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