Assay of vitamin b in12 xx

Details Assay of vitamin b in12 xx Assay of vitamin b in12 xx

G - Physics

01

N

167/40, 260/210.

G01N 33/82 (2006.01) C07H 23/00 (2006.01)

Patent

CA 1180273

ABSTRACT Vitamin B12 in liquid samples, such as human serum or human plasma, is assayed by a competitive binding technique using intrinsic factor and certain labelled vitamin B12 derivatives. The labelled derivatives are formed from the (d)-monocarboxylic acid isomer of vitamin B12, which isomer is free from other monocarboxylic acid isomers (and derivatives thereof) of vitamin B12, by binding to the (d)-isomer, via the carboxylic group, a compound which is itself a label (e.g. an enzyme) or which comprises a label (e.g. a fluorophore), or to which a label is attached (e.g. a histidine ester to which 125I is attached). The labelled derivatives of the (d)-monocarboxylic acid of vitamin B12, free from other isomeric monocarboxylic acids and derivatives, are novel and constitute one aspect of the invention.

403150

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