Method and reagent system for four-population differential...

Details Method and reagent system for four-population differential... Method and reagent system for four-population differential...

G - Physics

01

N

150/11, 150/3

G01N 1/28 (2006.01) G01N 33/48 (2006.01) G01N 33/50 (2006.01)

Patent

CA 1248856

ABSTRACT This invention describes a reagent system, including saponin in a lysing reagent and a rapidly active cross-linking agent such as glutaraldehyde as a fixing reagent, which reproducably affects whole blood to cause the red blood cells to stromatolyze and modifies the leukocytes to generate data to define four distinct clusters for detection and classification by flow analysis instrumentation. The clusters represent the four major leukocyte types found in blood: lymphocytes, monocytes, neutrophils and eosinophils, thus providing a method of leukocyte differential analysis. The parameters used for the leukocyte classification include combinations of two or more of DC (Coulter) volume, high frequency (RF) size, opacity (RF size/DC volume), light scatter at various angular ranges, and fluorescence at various wavelengths of illumination.

482841

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