Method of regenerating soybeans from cultured soybean...

Details Method of regenerating soybeans from cultured soybean... Method of regenerating soybeans from cultured soybean...

Category

A - Human Necessities

Subcategory

01

Subsubcategory

G

CPC

47/4

IPC codes

A01G 1/00 (2006.01) C12N 5/00 (2006.01) C12N 5/04 (2006.01)

Type

Patent

Patent number

CA 1268631

Description

A METHOD OF REGENERATING SOYBEANS FROM CULTURED SOYBEAN COTYLEDONARY NODES ABSTRACT The present invention is directed to a method of regenerating soybean plants from callus culture. Briefly, the method comprises the following steps: a. germinating soybean seeds on nutrient medium containing a cytokinin to produce a donor plant; b. excising the cotyledonary node region from said donor plant; c. culturing the excised nodal tissue on a nutrient medium containing a cytokinin until callus tissue develops which contains shoots; and d. removing said shoots from said callus and rooting said shoots in nutrient medium free from exogenous hormone to form a plantlet.

Application Number

468494

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