Process for purifying proinsulin-like materials

Details Process for purifying proinsulin-like materials Process for purifying proinsulin-like materials

C - Chemistry, Metallurgy

07

K

530/7.4

C07K 14/62 (2006.01) C07K 1/14 (2006.01)

Patent

CA 1284249

Abstract A process is provided for separating impuri- ties from an impure mixture containing proinsulin-like material, which comprises: (1) applying said mixture to a reverse phase macroporous acrylate ester copolymer resin support at a pH of from about 7 to about 10; and (2) eluting said proinsulin-like material from said support with an aqueous eluant having a pH of from about 8 to about 11 and containing from about 10% to about 30% by volume of an organic diluent selected from the group consisting of acetone, aceto- nitrile, and a combination of acetone and acetonitrile.

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