Reagent strip test for antithrombin-iii

Details Reagent strip test for antithrombin-iii Reagent strip test for antithrombin-iii

C - Chemistry, Metallurgy

12

Q

150/11

C12Q 1/56 (2006.01) C12N 9/99 (2006.01) G01N 33/52 (2006.01) G03F 7/20 (2006.01)

Patent

CA 1187387

ABSTRACT OF THE DISCLOSURE Disclosed is a method and device for the quan- titative determination of AT-III in mammalian blood plasma. The method involves contacting a plasma sample containing excess heparin with a 3 layered reagent strip comprising: i. a first upper layer of a bibulous material con- taining thrombin and a buffer; ii. a second lower layer of a bibulous material containing a fluorogenic or chromogenic sub- strate capable of interacting with thrombin in a time related chemical manner; and iii. a bottom layer of water impermeable material. The chemical change in the strip is determined as a function of time and this change is compared with that obtained in samples containing known amounts of AT-III.

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