Method of determining a biological substance involving...

Details Method of determining a biological substance involving... Method of determining a biological substance involving...

G - Physics

01

N

167/43, 150/8

G01N 33/533 (2006.01) G01N 33/58 (2006.01)

Patent

CA 1314464

ABSTRACT A biological substance is determined with the use of a marker comprising a lanthanide or other metal ion coupled to the biological substance by means of a chelate- forming compound. The chelate complex is dissociated by reduction of the ambient pH to a sufficiently low level to liberate the metal ion and the metal ion is then che- lated with a separate chelating chromophore with an increase in the pH to a value closer to the optimum for determination of the metal-chelated chromophore by fluor- escence spectroscopy, luminometry, colourometry or the like.

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