C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/15.5
C12Q 1/48 (2006.01) C12Q 1/50 (2006.01)
Patent
CA 1199858
ABSTRACT OF THE DISCLOSURE A method of determining dTk isoenzyme levels of a human or animal body fluid or cell sample is disclosed herein. The method includes the steps of reacting the sample with a substrate for the isoenzyme in the presence of a phosphate donor and a buffer system. The amount of phosphorylated product formed is measured, that amount being proportional to the isoenzyme level. The method uses (a) a buffer system at a pH ranging from 5 to 9 and which is present in a concentration of no more than 250 mM, (b) a substrate consisting of 2'-deoxy-5-halouridin, part of which has a radio-labelled 5-halogen, and which is present in a concen- tration ranging from 2 x 10-9 to 5 x 10-6M, and (c) the phosphate donor which is present in a concentration not exceeding 20 mM. The sensitivity of the method permits measurement of minute amounts of dTk. The method can be used for, e.g., the diagnosis, prognostics and monitoring of diseases involving altered ATP mediated dTk activity, e.g., cancers, tumours, and certain virus infections or altered CTP mediated dTk activity, e.g., HSV type 1, type 2, and VZV infections.
428172
Gronowitz Jan-Simon
Kallander Clas F.r.
Borden Ladner Gervais Llp
Gronowitz Jan-Simon
Kallander Clas F.r.
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