C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/15.1
C12Q 1/28 (2006.01)
Patent
CA 1201369
CATALYZED COLORIMETRIC AND FLUOROMETRIC SUBSTRATES FOR PEROXIDASE ENZYME DETERMINATIONS Abstract of the Disclosure Improved, catalyzed substrates for use in developing characteristic colors or florescence in the presence of peroxidase enzymes (e.g., horseradish peroxidase) are disclosed which in- clude as a rate accelerator a substituted phenol such as a p-halogenated phenol. The complete substrate typically includes a peroxide type oxidizing agent (e.g., hydrogen peroxide), a chromogenic or flurogenic compound (e.g., ABTS), a buffer and the accelerator compound. Advanta- geously, the accelerator should provide at least about 50 percent rate enhancement for the sub- strate, as compared with an otherwise identical, accelerator-free substrate reacted under the same conditions; however, the most preferred accelera- tor, p-iodophenol, gives enhancements on the order to 1,000 percent. The substrates are particularly useful in so-called ELISA determinations which involve an enzyme-linked moiety, and permit detec- tion at very low concentration levels unobtainable with conventional colorimetric substrates.
437225
Enzyme Technology Company Partnership
Gowling Lafleur Henderson Llp
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