Homogeneous polynucleotide displacement assay method, kit...

Details Homogeneous polynucleotide displacement assay method, kit... Homogeneous polynucleotide displacement assay method, kit...

C - Chemistry, Metallurgy

12

Q

150/3.3, 150/8.5

C12Q 1/68 (2006.01) C12Q 1/48 (2006.01)

Patent

CA 1272939

ABSTRACT HOMOGENEOUS POLYNUCLEOTIDE DISPLACEMENT ASSAY METHOD, KIT AND REAGENT COMPLEX An RNA signal strand is displaced from a probe strand by a target nucleotide sequence. Without separation, a digestion enzyme selective for displaced RNA, e.g., polynucleotide phosphorylase, digests the displaced RNA to nucleoside phosphates including ADP. The digestion products are detected, e.g. by phosphorylating the ADP to ATP and detecting the ATP by bioluminescence.

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