Microorganism identification technique

C - Chemistry – Metallurgy – 12 – Q

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73/51, 150/16.4

C12Q 1/04 (2006.01) C12Q 1/16 (2006.01)

Patent

CA 1202234

"Microorganism Identification Technique" Abstract A method for the identification of micro-organisms comprises adding to a sample containing an un- known micro-organism an emissive agent such as a radio- active amino acid to produce a mix of emissive products that depends on the metabolic mechanism of the micro- organism. After incubation, the reaction is arrested and the emissive products are separated, as by electro- phoresis on a gel plate. The plate may then be autora- diographed by exposure to a photographic film to produce on the latter a characteristic band pattern functioning as an identifier for the micro-organism. Identification can be effected by comparing the identifier for the unknown with a collection of identifiers for known micro- organisms to find a match with one of these known identifiers. The comparison may be carried out by scanning the unknown identifier to produce a signal which is compared with signals representing known identifiers stored in a computer. Alternatively, the emissive products, after separation, may be detected by direct scanning to provide an identifier signal for computer processing.

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