Streptokinase-coding recombinant vectors

Details Streptokinase-coding recombinant vectors Streptokinase-coding recombinant vectors

C - Chemistry, Metallurgy

12

N

195/1.14, 195/1.

C12N 15/00 (2006.01) C07K 14/315 (2006.01) C12N 1/20 (2006.01) C12N 9/10 (2006.01)

Patent

CA 1223223

ABSTRACT A recombinant vector adapted for transformation of a suitable host to produce streptokinase is disclosed, said recombinant vector comprising a vector into which a polydeoxy- ribonucleotide fragment which codes for the synthesis and secretion of streptokinase has been inserted. Nucleotide sequences encoding for secretion and synthesis of strepto- kinase are also disclosed, as well as a process for preparing the recombinant vector comprising: digesting a vector with a restriction endonuclease to produce linear vector DNA; and ligating the linear vector DNA with a polydeoxyribonucleotide fragment which codes for synthesis and secretion of streptokin- ase, to produce a recombinant vector comprising a vector into which a polydeoxyribonucleotide fragment which codes for the synthesis and secretion of streptokinase has been inserted.

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