Excretion of heterologous protein from e. coli

C - Chemistry – Metallurgy – 12 – N

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195/1.2, 195/1.2

C12N 15/70 (2006.01) C12N 1/21 (2006.01) C12N 15/11 (2006.01)

Patent

CA 1332813

Excretion of heterologous proteins from E. coli hosts is achieved by transforming the host cells with a recombinant DNA construct which comprises a control. region and a coding region in which the DNA coding for the heterologous protein is coupled in reading frame with the DNA coding for the ompA signal peptide, the control region comprising the tac promoter, the lac operator and a consensus ribosome binding site.

609445

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