Immunoassay for human chorionic gonadotropin

Details Immunoassay for human chorionic gonadotropin Immunoassay for human chorionic gonadotropin

G - Physics

01

N

167/139, 167/37

G01N 33/577 (2006.01) G01N 33/574 (2006.01) G01N 33/76 (2006.01)

Patent

CA 1229549

IMMUNOASSAY FOR HUMAN CHORIONIC GONADOTROPIN Abstract of the Disclosure An immunoassay for detecting and measuring hCG in a sample includes an antibody directed to the carboxy terminal portion of the .beta. subunit of hCG and a monoclonal antibody directed to a determinant on hCG at a locus sufficiently remote from the carboxy terminal portion of the .beta. subunit of hCG that both antibodies can simultaneously bind to hCG, wherein at least one of the antibodies is detectable when both are bound to hCG. In a presently preferred embodiment, an immunoassay for hCG or hCG .beta. in urine includes a purified, labeled or detectable serum-derived antibody directed to the car- boxy-terminal portion of the .beta. subunit of hCG and a matrix- bound monoclonal antibody directed to a locus on the .beta. subunit sufficiently remote from the carbox-terminal portion that both antibodies can simultaneously bind to hCG or hCG .beta..

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