Stabilization of coenzymes in aqueous solution

Details Stabilization of coenzymes in aqueous solution Stabilization of coenzymes in aqueous solution

C - Chemistry, Metallurgy

12

N

150/15, 150/3.2

C12N 9/96 (2006.01) C12P 1/00 (2006.01) C12Q 1/00 (2006.01) C12Q 1/48 (2006.01) C12Q 1/58 (2006.01)

Patent

CA 1178875

Abstract of the Disclosure A stabilized aqueous coenzyme solution for use in the clinical assay of a selected biological constituent through the enzymic reaction of an enzyme with a selective substrate for the enzyme and n coenzyme. The aqueous coenzyme solution comprises at lcast onc first enzyme and a first substrate for selectively roacting with the first enzyme in the assay, a coenzyme for interacting with the first enzyme and first substrate in the assay which coenzyme degrades forming a conversion conversion product, and an enzyme and substrate system comprises at for regenerating the coenzyme from the coenzyme conversion product. The enzyme and substrate system comprises at least one second enzyme and second substrate selective for the second enzyme which react with the coenzyme conversion product to regenerate the coenzyme. A kit for performing the diagnostic assay is also disclosed.

366093

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