Apparatus and process for immunoassays

Details Apparatus and process for immunoassays Apparatus and process for immunoassays

G - Physics

01

N

167/44

G01N 33/53 (2006.01) G01N 33/543 (2006.01)

Patent

CA 1288690

ABSTRACT Disclosed is an improved apparatus for conducting immunoassays. The apparatus comprises a container, and a test zone to which is bound an antibody, typically a monoclonal antibody, or which is capable of extracting cells from a fluid sample. The test zone may be membrane, filter or a porous matrix in which microspheres, to which are bound antibody, are entrapped The apparatus further comprises a liquid absorbing zone which is composed of adsorbent material which acts, when in contact with the test zone, to induce flow through the test zone when a fluid sample is added to it. At least one vent port in communication with the liquid absorbing zone and the opening of the container is provided to allow gas displaced by the addition of fluid to be discharged from the container. The flow of the displaced gas in a direction opposite to the flow of assay reagents through the liquid absorbing zone. Accordingly, the container can be sealed entirely from the environment by the use of a single removable seal over the opening of the container.

541342

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