Device and method for detecting antigens and antibodies

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/58 (2006.01) B01L 3/02 (2006.01) G01N 33/535 (2006.01) G01N 33/543 (2006.01) G01N 35/00 (2006.01) G01N 35/10 (2006.01)

Patent

CA 1184847

ABSTRACT OF THE DISCLOSURE A device for use in detecting or determining the presence of antigenic or haptenic substances or antibodies in a sample comprises a plurality of tubular or capillary elements, each having antibodies or antigenic or haptenic substances attached to an internal surface thereof, and means for causing fluids to pass simultaneously or sequentially through the plurality of capillary elements. A method and test kit for detecting and determining the presence of antigenic or haptenic substances or antibodies in a sample by the enzyme-linked immunosorbent assay technique is characterised by use of urease as the enzyme in an antibody-enzyme or antigen-enzyme conjugate, with urea being used as the enzyme substrate and the presence of ammonia being detected or determined using di-bromo o-cresolsulfonphthalein.

392770

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