Method to produce immunodiagnostic reagents

Details Method to produce immunodiagnostic reagents Method to produce immunodiagnostic reagents

G - Physics

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G01N 33/53 (2006.01) C07K 7/06 (2006.01) C07K 7/08 (2006.01) C07K 16/00 (2006.01) C07K 16/30 (2006.01) G01N 33/543 (2006.01) G01N 33/68 (2006.01) G01N 30/02 (2006.01)

Patent

CA 1340459

Screening methods to obtain suitable antibodies for use in immunoassays for analytes not ordinarily susceptible to detection by this means involves in vitro screening of panels of cells secreting a representative selection of antibodies. An application of this method also permits the preparation of specific mimotopes which mimic the immunological activity of the desired analyte, which mimotopes can then be used as competitors in the immunoassay or can be used to immunize subject mammals in order to improve the specificity and affinity of the antibodies. Methods to identify a particular analyte by its pattern of binding strength to a panel of related antibodies and to match an arbitrary analyte with an immunoreactive member of a panel of candidate antibodies are also disclosed.

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