C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
167/139, 167/44,
C07K 17/02 (2006.01) C07K 1/22 (2006.01) C07K 7/06 (2006.01) C07K 7/08 (2006.01) C07K 14/81 (2006.01) C07K 16/40 (2006.01) C12N 9/74 (2006.01) G01N 33/68 (2006.01) G01N 33/86 (2006.01)
Patent
CA 1339027
The invention relates to synthetic peptides which have amino acid sequences which correspond, in whole or in part, to the amino acid sequence of prothrombin and are anti- genic, to the use thereof for the immunization of an ani- mal and for the purification of specific antibodies, to antibodies against these peptides, and to the use of the antibodies for the determination of the fragments F2/F1+2. The antibodies hitherto used for the determination of the content of the fragments F2/F1+2 have been induced by immunization with natural, highly purified prothrombin fragments F2/F1+2. The isolation of these prothrombin fragments is elaborate and costly, and the antibodies gene- rated therewith show cross-reactions with intact prothrom- bin. Used for the immunization according to the invention are synthetic peptides which have amino acid sequences which correspond, in whole or in part, to the amino acid sequence of prothrombin and are antigenic. The resulting antibodies react specifically with the antigen used for the immunization; if the peptides contain the carboxyl- terminal amino acid sequence of the fragments F2/F1+2 resulting after cleavage of the prothrombin molecule with factor Xa, then the antibodies generated react specific- ally with the fragments F2/F1+2 but not with intact natural prothrombin, so that information can be gained about the degree of activation of prothrombin.
575049
Pelzer Hermann
Stuber Werner
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Siemens Healthcare Diagnostics Products Gmbh
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