Method of somatotropin solubilization and naturation

Details Method of somatotropin solubilization and naturation Method of somatotropin solubilization and naturation

C - Chemistry, Metallurgy

07

K

530/7.26

C07K 14/61 (2006.01) C07K 1/113 (2006.01)

Patent

CA 1268300

METHOD OF SOMATOTROPIN SOLUBILIZATION AND NATURATION ABSTRACT A method for solubilization and naturation of somatotropin protein from refractile bodies of host cells is disclosed. The method embraces the discovery that an aqueous urea or dimethylsulfone solution can be effectively used to solubilize refractile bodies containing such somatotropin protein. Once solubilized, somatotropin protein can be natured in a urea or dimethylsulfone solution by contacting the solution with a mild oxidizing agent for a time sufficient to result in the formation of the disulfide bonds. Naturation can efficiently occur even at high protein concentration, in an impure preparation and in the absence of reducing agent.

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