G - Physics – 01 – N
Patent
G - Physics
01
N
167/37
G01N 33/92 (2006.01) G01N 33/536 (2006.01)
Patent
CA 1126651
Abstract of the disclosure: The invention relates to a process for the quantitative determination of a serum protein, especially apolipoprotein, in turbid serum or plasma samples by nephelometry, which comprises, after having carried out an immunologic reaction with the corresponding antibody, measuring in the presence of a tenside of the formula CH3-(CH2)m-R-(CH2-CH2-O)n-H (I) in which R is -0-? -NH-; -CH=CH-(CH2)x-NH; -N (CH2-CH-OH)-O-?-O; y is H- or CH3-(CH2)q-,m and q, being identical or dirferent, each are an integer of from 6 to 26. preferably 6 to 18; n is an integer of from 7 to 50, preferably 7 to 25; and x is 7 or 8; the tenside being used in a concentration of from 10-3 to 10-1 % by volume. Alternatively, the measures can be taken after enzymatic degradation of the lipids and subsequent immunologic reac- tion with the corresponding antibody in the presence of a nonionic tenside of the formula (I), in which R is as de- fined above or -O-; -S-; -?-O; -CH=CH-(CH2)x-O-; -?-O-, R' being CH3 or C2H5, m, n and x are as derined in claim 1. Further subjects of the invention are the use of a tenside of the cited formula for serum work-up ror quantitative determination of an apolipoprotein, and an agent for serum work-up for quantitative determination Or an apolipopro- tein, consisting of a mixture of at least one lipid-degrad- ing enzyme and a tenside of the cited formula.
331145
Behringwerke Aktiengesellschaft
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
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