Process for the preparation of a glucose-free hemoglobin...

Details Process for the preparation of a glucose-free hemoglobin... Process for the preparation of a glucose-free hemoglobin...

G - Physics

01

N

167/114, 150/3

G01N 33/66 (2006.01) G01N 33/72 (2006.01)

Patent

CA 1210694

11 ABSTRACT OF THE DISCLOSURE A process for the preparation of a glucose-free glycosylated hemoglobin standard in which hemoglobin with a known content of glycosylated hemoglobin HbAIa+b+c is converted to cyanmethemoglobin by means of a K3(FeCN)6/KCN solution, stabilized with a sodium phosphate-cyanide buffer and preserved by lyophilizing at -40°C with a subsequent increase in temperature to -20°C under a high vacuum and stored at this temperature with the exclusion of light, whereupon the glucose-free glycosylated hemoglobin standard may be reconstituted by the addition of an aqueous medium.

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