Tissue protein pp.sub.4, a process for isolating it and its use

C - Chemistry – Metallurgy – 07 – K

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167/104, 530/9,

C07K 16/18 (2006.01) C07K 14/47 (2006.01)

Patent

CA 1224775

-1- Abstract of the Disclosure The protein PP4 which has a) an electrcphoretic mobility in the range between that of ?1 and ?2 globulins; b) an isoelectric point of 4.85 + 0.15; O c) a sedimentation coefficient s 20 w of 3.3 ~ 0.2S; d) a molecular weight determined in polyacrylamide gel containing sodium dodecylsulfate (DSD) of 35,000 + 5,000; 1% e) an extinction coefficient E 1cm (280 nm) of 5.9 + 0.6; f) a carbohydrate content of 2.4 + 0.94% (g/100 g) (man- nose 0.3 + 0.2%, galactose 0.4 + 0.2%, xylose 0.1 + 0.4%, glucose 0.2 0.1%, glucosamine 1.0 + 0.2% and neuraminic acid 0.4 + 0.2%) and g) a specified aminoacid composition, and a process for its preparation are described. PP4 can be used to pre- pare antisera which can be employed to detect and deter- mine PP4 in body fluids in order to diagnose diseases of particular organs, as a "marker" to monitor the progress of a disease or to check therapy.

452691

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