Hybridization assay employing labeled pairs of hybrid...

Details Hybridization assay employing labeled pairs of hybrid... Hybridization assay employing labeled pairs of hybrid...

C - Chemistry, Metallurgy

12

Q

167/37, 150/8.5

C12Q 1/68 (2006.01) G01N 33/52 (2006.01) G01N 33/53 (2006.01)

Patent

CA 1239580

ABSTRACT OF THE DISCLOSURE A nucleic acid hybridization assay wherein the hybrid formed with the probe has binding sites for two specific binding reagents, one of which comprises a first label and the other a second label, interaction of the first and second labels providing a detectable response which is measurably different when the two labeled reagents are both bound to the same hybrid compared to when the two labeled reagents are not so bound. The formation of the hybrid assay product brings the two labels within a proximate interaction distance of one another, e.g., as in the cases of sequential catalyst (enzyme) interaction and energy transfer. Since the labels provide a response which is distinguishable when they are associated with a hybridized probe, no separation step is required.

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