C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/128, 195/39,
C12N 9/50 (2006.01) C12N 9/52 (2006.01)
Patent
CA 1172979
ABSTRACT The present invention provides endoproteinase- Lys-C from bacteria, consisting of a chain of molecular weight 35000 to 3o000 Dalton, having a pH optimum at pH 7.7 and being inhibited by aprotinin but not being inhibited by alph2-macroglobulin, .alpha.1-antitrypsin and ethylenediamine-tetraacetic acid. The present invention also provides a process for obtaining this enzyme, wherein a culture broth of an appropriate bacterial strain which has been filtered or pre-purified by usual methods is chromato- graphed over carrier-fixed alpha2-macroglobulin-metal complex and the enzyme is obtained from the filtrate.
383349
Schrenk Jurgen
Wunderwald Peter
Boehringer Mannheim G.m.b.h.
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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