Method of making a selected protein

Details Method of making a selected protein Method of making a selected protein

C - Chemistry, Metallurgy

12

N

195/1.14, 195/1.

C12N 15/00 (2006.01) C07K 14/62 (2006.01) C12N 9/86 (2006.01) C12N 15/66 (2006.01) C12P 21/02 (2006.01)

Patent

CA 1215920

METHOD OF MAKING A SELECTED PROTEIN ABSTRACT OF TEE DISCLOSURE A plasmid or phage gene for a periplasmic or extracellular bacterial protein is cleaved, a double- stranded DNA sequence coding for a selected protein or portion thereof from a eukaryotic cell such as insulin is inserted in that cleaved gene by recombinant DNA techniques and used to transform a bacterium, and the excreted selected protein is collected.

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