Cold stable liquid creatinine reagent

Details Cold stable liquid creatinine reagent Cold stable liquid creatinine reagent

G - Physics

01

N

150/1

G01N 33/70 (2006.01)

Patent

CA 1309000

ABSTRACT A cold stable liquid reagent system suitable for use in a Jaffe method for the determination of creatinine comprises: (a) a homogeneous liquid picric acid component comprising picric acid in an amount of no more than about 35 mM, such that the picric acid component remains visibly homogeneous at a temperature between about 2 to about 8°C for 30 days or more; (b) a homogeneous liquid alkaline buffer component comprising: (i) an alkaline material in sufficient amount to maintain the pH of the alkaline buffer component at more than about 13, for controlling the pH of the assay ready reagent formed by combining the picric acid and alkaline buffer components; (ii) at least one pH buffer material; (iii) a detergent for reducing interference by proteins in the Jaffe method; and (iv) borate for reducing interference by carbohydrates in the Jaffe method; wherein the alkaline buffer components contains substantially no metallic cations such that the component remains visibly homogeneous at a temperature between about 2 to about 8°C for 30 days or more. Preferably the alkaline material is tetramethylammonium hydroxide, and the detergent is diethanolamine dodecyl sulfate.

569422

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