Process for production of insulin-like growth factor i

Details Process for production of insulin-like growth factor i Process for production of insulin-like growth factor i

C - Chemistry, Metallurgy

12

N

530/13, 195/132,

C12N 15/62 (2006.01) C07K 14/57 (2006.01) C07K 14/65 (2006.01) C12N 1/20 (2006.01) C12N 15/17 (2006.01) C12N 15/71 (2006.01)

Patent

CA 1297813

ABSTRACT OF THE DISCLOSURE Human insulin-like growth factor I (IGF-I) is produced by culturing an organism containing therein a plasmid which includes a synthetic IGF-I gene and a promoter gene upstream of and adjacent to the IGF-I gene to control expression of IGF-I gene, in which the synthetic IGF-I gene codes for the expression of IGF-I, wherein IGF-I consists 70 amino acids in the following sequence: Image and the promoter gene has no structural gene, and recovering IGF-I as a non fused peptide from the culture broth; IGF-I has insulin-like potency and stimulation potency of sulfate- uptake by cartilage.

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