Ascorbate-resistant broad range glucose test composition,...

C - Chemistry – Metallurgy – 12 – Q

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150/2, 150/15.1

C12Q 1/54 (2006.01) C12Q 1/28 (2006.01) G01N 33/66 (2006.01)

Patent

CA 1185154

ABSTRACT OF THE DISCLOSURE A composition, test device and method for detect- ing the presence and/or determining concentration of glucose in a liquid test sample are disclosed. The composition is capable of determining glucose con- centration over a broad range, e.g. 0 to about 5,000 milligrams (mg) of glucose per deciliter (dl) of test sample. Moreover, the composition is resistant to interference from the presence of ascorbate in the sample. The composition comprises glucose oxidase, peroxidase and, as an indicator capable of pro- ducing a detectable response ill the presence of pero- xidase and H2O2, a mixture of two compounds. One has the structure Image and the other has the structure Image in which R, same of different, is H or lower alkyl, R' is aryl and R" is lower alkyl.

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