Methods for producing a t7-type dna polymerase

Details Methods for producing a t7-type dna polymerase Methods for producing a t7-type dna polymerase

C - Chemistry, Metallurgy

12

N

195/123, 195/39,

C12N 15/54 (2006.01) C12N 9/12 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 1341512

A method of producing active T7-type DNA polymerase from cloned fragments. comprising placing the genes encoding said polymerase under the control of non-leaky promoters in a single cell, inducing expression of said genes when said cell is in logarithmic growth phase, or stationary phase, and isolating said polymerase from said cell. Preferably one of the genes is under the control of a promoter requiring T7 RNA polymerase for expression.

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