Methods of concentrating ligands and active membranes used...

Details Methods of concentrating ligands and active membranes used... Methods of concentrating ligands and active membranes used...

C - Chemistry, Metallurgy

12

N

195/33, 23/375,

C12N 11/08 (2006.01) A61K 39/29 (2006.01) B01D 67/00 (2006.01) B01D 69/14 (2006.01) C07K 16/00 (2006.01) G01N 33/543 (2006.01) G01N 33/545 (2006.01)

Patent

CA 1249781

Abstract: A chemically active membrane having a large surface area is provided in which a hydrophilic, microporous, skinless, polyamide membrane is chemi- cally bound to a residue of an activating agent which is capable of reacting with a biologically active material. The chemically active membrane, formed by react- ing a hydrophilic, microporous, skinless, polyamide membrane with an activating agent may be used to prepare a biologically active membrane having a large surface area which comprises an acceptor molecule such as a monoclonal antibody, a polyclonal antibody, an antigenic substance, a glycoprotein, Protein A, a lectin, a carbohydratet an enzyme substrate, a cofac- tor, an inhibitor, a hormone, an IgG class of immuno- globulin, a carrier protein, a receptor, heparin, a coagulation factor, or a histone covalently bound to the hydrophilic, microporous, skinless, polyamide membrane by reacting the chemically activate membrane with the acceptor molecule.

488217

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